Newborn litters of GF and SPF NMRI mice were placed and raised in
special plastic isolators until they reached 8 to 10 wk of age (Core Facility for
Germ Free Research, Karolinska Institutet). Only male animals were used in
these experiments. Animals were maintained on autoclaved R36 Lactamin
Chow (Lactamin) and kept in 12-h light cycles. All procedures were approved
by the Local Research Ethics Committee.

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